Journal: BMC Microbiology
Article Title: Repurposing Etalocib suppresses multidrug-resistant Staphylococcus aureus by disrupting the bacterial membrane
doi: 10.1186/s12866-025-04163-5
Figure Lengend Snippet: Inhibitory and bactericidal effects of Etalocib on the biofilm. A - C Effect of sub-inhibitory concentrations (1/8 ×, 1/4 ×, and 1/2 × MIC) of Etalocib on the biofilm formation of MSSA ( A ), MRSA ( B ) and E . faecalis ( B ) by crystal violet staining. The MIC values of Etalocib against EF16C168 were 3.13 μM. The MIC values of Etalocib against SA113, CHS101, CHS727, YUSA139, EF16C166, EF16C350 and EF16C353 were 6.25 μM. The MIC values of Etalocib against YUSA143, YUSA145 were 12.5 μM. The MIC values of Etalocib against YUSA21, YUSA50 were 25 μM. The MIC values of LZD against CHS727, YUSA145 and YUSA21 were 1 μg/mL. The MIC values of LZD against YUSA143, YUSA139, CHS101, SA113, YUSA50, EF16C166, EF16C168, EF16C350 and EF16C353 were 2 μg/mL. LZD, Linezolid. D - I Impact of different concentrations of Etalocib on S. aureus YUSA145 embedded in the mature biofilm was investigated by CLSM. Living cells and dead cells were labeled with SYTO 9 (green) and PI (red) dyes, respectively. Van: Vancomycin, as a positive control. Scale bar = 20 μm. For ( A ), n = 3 and data were presented by mean ± SD, two-way ANOVA with Dunnett’s multiple comparisons test. * P < 0.05, ** P < 0.01, *** P < 0.001, *** P < 0.0001
Article Snippet: The reference strain S. aureus SA113 (ATCC35556) purchased from American Type Culture Collection (ATCC), and was used as the quality control of antimicrobial susceptibility strain.
Techniques: Staining, Labeling, Positive Control